{"id":"9c6a3ae5279b","type":"article","url":"https://hartvaat.nl/2026/05/06/circulerend-mir-10b-5p-voorspelt-af-recidief-na-ablatie/","title":"Circulerend miR-10b-5p voorspelt AF-recidief na ablatie","title_en":"","category":"atriumfibrilleren","category_label":"Atriumfibrilleren","professions":["cardioloog"],"tags":[],"journal":"Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology","doi":"10.1093/europace/euag097","source_url":"https://doi.org/10.1093/europace/euag097","authors":["Εmmanouil P Vardas","Evangelos Oikonomou","Stylianos Tzeis","Panagiotis Theofilis","Maria Gazouli","Eleni Papadogeorgaki","Konstantinos Belogiannis","Dimitrios Asvestas","Emmanouil Vavouris","Dimitrios Charitos","Dimitris Tousoulis"],"significance":5,"published":"2026-05-06","source_date":"2026-05-06","image":"","kennis":["https://hartvaat.nl/kennis/atriumfibrilleren/elektrisch-remodellering-atrium/","https://hartvaat.nl/kennis/atriumfibrilleren/wat-is-atriumfibrilleren/"],"congress":"","summary_en":"A two-phase translational study identified circulating microRNAs associated with atrial fibrillation recurrence after catheter ablation. The discovery phase (n=29) flagged 14 miRNAs significantly linked to recurrence; in the prospective validation cohort (n=126), miR-10b-5p showed the highest discrimination (AUC=0.96). Lower miR-10b-5p expression independently predicted recurrence and substantially improved the baseline clinical model (ΔR² 63.3%). Pathway analysis linked miR-10b-5p to FOXO, p53 and circadian signalling — relevant to atrial remodelling. Multicentre validation is needed for clinical use.","created":"2026-07-03T10:32:51Z","updated":"2026-07-03T13:31:47Z","licence":"Citeer vrij, met bronvermelding en een link naar hartvaat.nl (de url van het record). Samenvattingen zijn redactioneel werk van HartVaat; de oorspronkelijke publicaties blijven van hun uitgevers (doi). Geen medisch advies.","body_markdown":"Tweefasige translationele studie identificeerde circulerende microRNA's geassocieerd met atriumfibrilleren-recidief na katheterablatie. In de discovery-fase (n=29) waren 14 miRNA's significant geassocieerd met recidief; in de prospectieve validatiecohort (n=126) toonde miR-10b-5p de hoogste discriminatie (AUC=0,96). Lagere miR-10b-5p-expressie was onafhankelijk geassocieerd met recidief en verbeterde de baseline-klinische predictie aanzienlijk (ΔR² 63,3%). Pathway-analyse koppelde miR-10b-5p aan FOXO-, p53- en circadiane signaalroutes — relevant voor atriale remodelering. Multicenter-validatie nodig voor klinische toepassing.","abstract_original":"AIMS: The identification of reliable biomarkers for atrial fibrillation (AF) recurrence post-catheter ablation remains a clinical challenge. This study aimed to identify circulating microRNAs (miRNAs) associated with post-ablation AF recurrence and examine their underlying molecular pathways using an integrative translational approach. METHODS AND RESULTS: This two-phase study included a discovery case-control phase (n = 29) followed by a prospective validation cohort (n = 126). In the discovery phase, 84 miRNAs were quantified via real-time PCR, and candidates were selected using differential expression analysis and machine learning. In the validation phase, five candidate miRNAs (hsa-miR-342-3p, hsa-miR-424-5p, hsa-miR-486-5p, hsa-miR-10b-5p, and hsa-let-7d-5p) were further evaluated to assess their prognostic performance. Pathway enrichment analysis was performed for the most predictive miRNA. Differential expression analysis identified 14 miRNAs to be significantly associated with AF recurrence. In the validation cohort, hsa-miR-10b-5p showed the highest discriminative performance (AUC = 0.96, P < 0.001). Multivariable logistic regression confirmed that lower expression of hsa-miR-10b-5p was an independent predictor of recurrence (OR = 0.06, P < 0.001), significantly improving the baseline clinical model (ΔR = 63.3%). Pathway analysis linked hsa-miR-10b-5p to FOXO signalling, p53 signalling, circadian rhythm and cellular senescence, pathophysiologic mechanisms that are critical to atrial remodelling, and fibrotic persistence. CONCLUSION: Down-regulation of circulating hsa-miR-10b-5p was independently associated with AF recurrence after catheter ablation and improved risk discrimination beyond clinical variables. These findings support its potential role as a prognostic biomarker, although further multicentre validation is required before clinical application."}