Peptidedeformylase reguleert aldosteronproductie via calbindine 1

BACKGROUND:Aldosterone-producing adenoma is a major cause of primary aldosteronism. However, the molecular mechanisms underlying aldosterone overproduction remain incompletely understood. The expression of peptide deformylase, which is essential for the maturation of mitochondrially encoded proteins, was significantly upregulated in our previous proteomic analysis. We aimed to elucidate the role of peptide deformylase in aldosterone overproduction.METHODS:Peptide deformylase expression was validated in enlarged aldosterone-producing adenoma samples harboring different mutations and in adrenocortical cell lines. A key protein exhibiting the most significant change was identified through proteomic analysis of peptide deformylase-knockdown cells and validated in vitro.RESULTS:Both peptide deformylase expression and aldosterone synthase (CYP11B2 [cytochrome P450 family 11 subfamily B member 2]) colocalization were significantly enhanced in aldosterone-producing adenoma samples. Among the 4