SerpinB1 beschermt tegen hypoxie-geïnduceerde pulmonale hypertensie via macrofaag-PASMC-crosstalk

Hypertension, Volume 83, Issue 3, Page e25832, March 1, 2026. BACKGROUND:Perivascular macrophages play a significant role in the pathogenesis of hypoxia-induced pulmonary hypertension via crosstalk to pulmonary artery smooth muscle cells (PASMCs) to stimulate their proliferation and pulmonary vascular remodeling. However, whether hypoxia exposure of macrophages affects cellular crosstalk remains entirely unclear. This study aimed to decipher the effects of hypoxia on macrophages’ crosstalk to PASMCs and elucidate the underlying molecular mechanisms.METHODS:Conditioned medium obtained from bone marrow-derived macrophages under normoxia or hypoxia was transferred for hypoxic culture of primary mouse PASMCs, followed by RNA sequencing analysis. Myeloid-specific SerpinB1-overexpressing mice were generated to explore SerpinB1’s role in macrophage inflammatory phenotype, PASMC proliferation, and pulmonary vascular remodeling.RESULTS:Hypoxia-exposure of macrophages produced a conspicuous augm